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LSM710 Spectral Confocal Microscope (Zeiss). This system consists of an Observer.Z1 inverted microscope for transmitted light and epifluorescence, coupled to a spectral confocal scanning unit equipped with six laser lines (405, 458, 488, 514, 561, and 633 nm). It is fitted with a CoolLED p300 LED illumination system. Available objectives are Plan-Apochromat 10x/0.45 DIC, 25x/0.8 Imm Korr DIC, 40x/1.3 Oil DIC, and 63x/1.40 Oil DIC. Image acquisition and analysis are performed using ZEN 2.3 SP1 software.
- Equipped with an IBIDI stage-top incubator providing controlled temperature, CO₂, and humidity conditions.
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Stellaris 8 Tau STED Spectral Confocal Microscope (Leica). Through STED technology, this system enables the study of subcellular structures with greater detail than that achievable with a conventional confocal microscope. It consists of a DMi8 inverted microscope for transmitted light and epifluorescence coupled to a spectral confocal system featuring a 405 nm laser, a white light laser (485–680 nm), and a 775 nm depletion laser. Available objectives are Plan-Apochromat 10x/0.40, 20x/0.75 multi-immersion DIC, 40x/1.3 Oil DIC, 63x/1.40 Oil DIC, and 100x/1.4 Oil STED White. The system is equipped with two HyD-S and one HyD-X hybrid spectral detectors. It includes both LIGHTNING and STED super-resolution modules, as well as the TauSense toolbox. Image acquisition and analysis are performed with LAS X software.
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Equipped with an OKOLAB incubation system providing controlled temperature, CO₂, and humidity conditions.
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Sample preparation guide: The Guide to STED Sample Preparation.
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Cell Observer Live-Cell Imaging System (Zeiss), consisting of an Observer.Z1 inverted microscope for transmitted light and epifluorescence coupled to an incubation system with controlled temperature, CO₂, and humidity conditions. The system is equipped with a high-resolution Prime BSI Express monochrome camera (Photometrics). Available objectives are Plan-Apochromat 10x/0.45, 20x/0.8, 40x/1.3 Oil DIC, and 63x/1.40 Oil DIC. Fluorescence illumination is provided by a Colibri 7 LED system with 385, 475, 555, 590, and 630 nm LEDs and a dual fluorescence filter slider. The system operates with ZEN 3.5 software, including the Deconvolution and Direct Processing image-processing modules.
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Nikon 90i Microscope, a motorized upright microscope for transmitted light and epifluorescence. It is equipped with a CoolLED p300 LED illumination system and filter sets for UV-2E/C DAPI, B-2E/C FITC, Y-2E/C Red, mCherry, and Cy5 HYQ. The microscope is fitted with two Nikon digital cameras: a cooled monochrome camera (DS-Qi1Mc) and a color camera for bright-field imaging (DS-Fi1). Image acquisition and analysis are performed using NIS-Elements 3.22 software. Objectives include Plan-Apochromat 4x/0.2, 20x/0.75, 40x/0.95, 60x/1.4 Oil, 100x/1.4 Oil, and Plan Fluor 10x/0.3. A Plan Achromat 2x/0.06 objective is available upon request.
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Axiophot Microscope (Zeiss), an upright transmitted light and epifluorescence microscope. It is equipped with filter sets for DAPI, green fluorescence, and red fluorescence. The system is coupled to an Olympus DP70 color camera with DP Controller imaging software. Available objectives are Achroplan 4x and Plan-Neofluar 10x/0.3, 20x/0.5, 40x/1.3 Oil, 63x/1.25 Oil, and 100x/1.3 Oil.
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Leica DMIL LED FLUO Inverted Microscope, for transmitted light and epifluorescence. It is equipped with a CoolLED p300 illumination system and FITC and Red filter sets. The microscope is coupled to a Nikon DS-2MBWc monochrome camera controlled by NIS-Elements F 3.0 software. Available phase-contrast objectives are 5x, 10x, 20x, and 40x. The system also includes an adapter for IMC (Integrated Modulation Contrast) imaging, similar to DIC, available upon request.
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Nikon SMZ Stereo Microscope.
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Zeiss SR Stereo Microscope.
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Image Analysis Workstation for microscopy image and data processing. Installed software includes NIS-Elements (Nikon), ImageJ/Fiji, ZEN Lite (Zeiss), LAS X (Leica), and AngioTool.