- The book Thyroid Hormones is an essential work on advanced protocols in the study of thyroid hormones
Constanza Contreras Jurado has contributed as editor in the publication of the book of protocols “Thyroid Hormones”, belonging to the renowned Springer Protocols series “Methods in Molecular Biology”. This work, structured in six parts, includes 16 detailed protocols related to thyroid hormones, their transporters and receptors, addressing a wide range of methodologies. From conventional techniques to the most advanced technologies, the book provides tools for in vitro and in vivo studies, using various cellular models, experimental animals and, in addition, chapters focused on clinical practice in humans.
Constanza Contreras Jurado completed a 10-year postdoctoral stay in the laboratory of Dr. Ana Aranda, at the Instituto de Investigaciones Biomédicas Sols-Morreale (IIBM), CSIC-UAM, during which she conducted in-depth research on thyroid hormones. Constanza is currently working in the Management area at the same center.
The book has benefited from the valuable collaboration of several members of this institute, such as Ana Aranda Iriarte, Ana Guadaño-Ferraz, Ana Montero Pedrazuela, Darío Cilleros Rodríguez, Marina Guillén Yunta, Marina Lasa and Soledad Bárez López, whose extensive experience in the subject has been fundamental in guaranteeing the quality and depth of the contents presented.
Chapter Index:
1. Thyroid hormones and co-workers: an overview.
2. Thyroid hormone action by genomic and nongenomic molecular mechanisms.
3. A sensitive radioimmunoassay for T3 and T4 determinations in plasma and tissues.
4. Generation of a mouse model for the study of thyroid hormones regulatory effect on the immune system.
5. Analysis of apoptosis by thyroid hormone induction.
6. Immunophenotyping of leukocytes in brain in hypothyroid mice.
7. Immunophenotyping of hematopoietic cells in the spleen in hypothyroid mice.
8. Mouse embryonic fibroblasts reprogramming to induced pluripotent stem cells by T3.
9. DNA methylation analysis by bisulfite pyrosequencing of mouse embryonic fibroblasts with reprogramming enhanced by thyroid hormones.
10. Analysis of the pattern of RNA expression in the skin of TR-deficient mice by RNA-seq.
11. miR-21, miR-31, miR-34a, and miR-203 changes validation by quantitative polymerase chain reaction in isolated keratinocytes.
12. Magnetic resonance imaging techniques for investigating the MCT8-deficient brain in murine disease models.
13. Experimentally manipulating the thyroid hormone axis in zebrafish.
14. Review of surgical interventions in the thyroid gland: recent advances and current.
15. Immunohistochemical study protocol in thyroid pathology.
16. Thyroid hormones testing in euthyroid and LT4-treated patients during covid-19 hospitalization.
If anyone would be interested in having a pdf copy of this book, please write to Constanza to request it at the following e-mail address: sccontreras@iib.uam.es